Towards Robot Scientists for autonomous scientific discovery
© Sparkes et al; licensee BioMed Central Ltd. 2010
Received: 14 July 2009
Accepted: 4 January 2010
Published: 4 January 2010
We review the main components of autonomous scientific discovery, and how they lead to the concept of a Robot Scientist. This is a system which uses techniques from artificial intelligence to automate all aspects of the scientific discovery process: it generates hypotheses from a computer model of the domain, designs experiments to test these hypotheses, runs the physical experiments using robotic systems, analyses and interprets the resulting data, and repeats the cycle. We describe our two prototype Robot Scientists: Adam and Eve. Adam has recently proven the potential of such systems by identifying twelve genes responsible for catalysing specific reactions in the metabolic pathways of the yeast Saccharomyces cerevisiae. This work has been formally recorded in great detail using logic. We argue that the reporting of science needs to become fully formalised and that Robot Scientists can help achieve this. This will make scientific information more reproducible and reusable, and promote the integration of computers in scientific reasoning. We believe the greater automation of both the physical and intellectual aspects of scientific investigations to be essential to the future of science. Greater automation improves the accuracy and reliability of experiments, increases the pace of discovery and, in common with conventional laboratory automation, removes tedious and repetitive tasks from the human scientist.
Towards the full automation of scientific discovery
A Robot Scientist encompasses a combination of different technologies: computer controlled scientific instruments, integrated robotic automation to link the instruments together, a computational model of the object of study, artificial intelligence and machine learning to iteratively create hypotheses about a problem and later interpret experimental results (closed-loop learning), and the formalisation of the scientific discovery process. We show how these elements come together to create an automated closed-loop learning system: a Robot Scientist.
Automation in all its forms has played an integral role in the development of human society since the 19th century. The advent of computers and computer science in the mid-20th century made practical the idea of automating aspects of scientific discovery, and now computing is playing an increasingly prominent role in the scientific discovery process [1, 2]. Experimental scientists use computers for instrument control, data acquisition and data analysis, and the functionality available on scientific instrumentation controlled by computers is improving rapidly. In addition, an increasing number of scientists no longer conduct physical experiments, instead using simulation or data-mining to discover new knowledge from existing data . Artificial intelligence (AI) has been used in an attempt to automate some of the intelligent aspects of the scientific discovery process still predominantly carried out by human scientists. Some examples of systems using AI components follow:
DENDRAL was an AI program developed in the 1960s that used background knowledge of chemistry to analyse experimental mass spectra data. It used heuristic search to determine solutions for the chemical structures responsible for the spectra, and was the first application of AI to a problem of scientific reasoning. This version became known as Heuristic-DENDRAL. A variant called Meta-DENDRAL followed, and was the first expert system for scientific hypothesis formation. It took a set of possible chemical structures and corresponding mass spectra as input, and inferred a set of hypotheses to explain correlation between some of the proposed structures and the mass spectrum. This information was then used to describe the knowledge that Heuristic-DENDRAL could utilise in its search for suitable structures .
AM was an Automated Mathematician, a heuristic artificial intelligence program that modelled mathematical discovery in the mid 1970s . It was said to have discovered numbers, prime numbers and several interesting mathematical conjectures. This system later evolved into EURISKO, developed in the late 1970s, which was more flexible in that it could be applied to other task domains. EURISKO was used successfully, for example, in optimising the design of integrated circuits for microchips .
KEKADA was a another heuristic based system that could develop hypotheses and plan experiments, searching for surprising phenomena . Kulkarni and Simon used this system to model the discovery of the urea synthesis pathway by Krebs. However, KEKADA had limited background knowledge when compared to human scientists, and like AM and EURISKO, needed more heuristics in order to continue its discoveries.
BACON , ABACUS , Fahrenheit  and IDS  were automated data driven discovery systems that could discover scientific laws as algebraic equations. They relied on data being entered by the experimenter, or on simulation of experiments. More recently, another example of a data driven system uses iterative cycles of algorithmic correlation to extract natural laws of geometric and momentum conservation, using data captured from motion-tracking experiments .
Most intelligent scientific discovery programs still do not 'close the loop' (feeding their results back into their experimental models) and do not design or execute their own experiments. Now, with recent advances in hardware and technology, the limitations of being unable to run experiments are diminishing. For example, microfluidics ('lab-on-a-chip') based approaches to experimentation may soon allow small controllable biological experimental systems to be linked to automated scientific discovery [12, 13]. We look forward to these advances in technology promoting future developments in automated scientific discovery. Integrated robotic systems are now capable of carrying out highly complex scientific discovery processes [14–18]. The majority of such automation is designed to perform experiments, previously conducted manually, in a more efficient, reliable and accurate manner than a human could ever achieve. Automation also enables previously impractical experiments to be carried out (such experiments may involve dangerous chemicals, pathogenic organisms, numerous assays or process steps, or need frequent measurements over long periods). With this type of system a human scientist typically sets-up the automated system to perform a sequence of processes, the system then executes the various steps automatically, and finally the human analyses the results. Any computation involved is usually connected with running the system, or with data management, visualisation and analysis. Complex databases are required to manage scientific data and knowledge and, as a result, science increasingly depends on efficient information management and manipulation .
Computational models or simulations have been used to give insight into how complex systems work; they represent the current state of understanding, provide a basis for predictions, and also have the benefit of being relatively cheap to execute. Models can be perturbed by making computational modifications to external conditions or to the architecture of the models themselves, then tested against acquired data. Such models can be constructed manually using human knowledge (e.g. ) or be automatically derived from experimental data (e.g. ).
The formal recording of scientific experimental data and meta-data can help towards creating better models, as well as facilitating the easier reuse of that data. Recording scientific data and meta-data in formal languages can provide complete, accurate, and detailed descriptions of why experiments were performed, how they were carried out, what the observed and derived results were, and how these results were interpreted. A well implemented formalisation provides the transparency required for science, allowing others to understand exactly why, as well as how an experiment was done, and provides all the essential information required to repeat that experiment.
To fully automate the scientific discovery process, computers also need to be able to create the initial hypotheses that define the reasons for carrying out the experiments, and then to be capable of learning from the results. Deduction, induction and abduction are types of logical reasoning used in scientific discovery . Deduction enables the inference of valid facts from existing known facts and rules, induction enables the inference of hypothesised rules from known facts, and abduction enables the inference of hypothesised facts from known facts.
The full automation of science requires 'closed-loop learning', where the computer not only analyses the results, but learns from them and feeds the resulting knowledge back into the next cycle of the process . Computational closed-loop learning systems have certain advantages over human scientists: their biases are explicit, they can produce full records of their reasoning processes, they can incorporate large volumes of explicit background knowledge, they can incorporate explicit complex models, they can analyse data much faster, and they do not need to rest.
The Robot Scientist concept
Robot Scientist prototypes
Here we describe our two prototype Robot Scientists, 'Adam' and 'Eve'. Adam has already proven itself by discovering new knowledge , whilst Eve is still under development. Both robots are designed to carry out biomedical scientific research.
A Robot Scientist to study yeast metabolism - 'Adam'
Our first prototype Robot Scientist, Adam, was physically commissioned at the end of 2005 (see Figure 2). It was designed to carry out microbial growth experiments to study functional genomics in the yeast Saccharomyces cerevisiae, specifically to identify the genes encoding 'locally orphan enzymes'. A locally orphan enzyme is an enzyme that is known to exist in an organism, but where the corresponding gene is as yet unidentified (definition agreed with Yannick Poullot and Peter D. Karp, who defined the term 'orphan enzyme' which has a slightly different meaning, see ). Adam uses a comprehensive logical model of yeast metabolism (based on the Forster iFF708 model), coupled with a bioinformatic database (Kyoto Encyclopaedia of Genes and Genomes - KEGG) and standard bioinformatics homology search techniques (PSI-BLAST and FASTA) to hypothesise likely candidate genes that may encode the locally orphan enzymes. This hypothesis generation process is abductive. There were two types of hypothesis generated. The first level links an orphan enzyme, represented by its enzyme class (E.C.) number, to a gene (ORF) that potentially encodes it. This relation is expressed as a two place predicate where the first argument is the ORF and the second the E.C. number. An example hypothesis at this level is:
The second level of hypothesis involves the association between a deletant strain, referenced via the name of its missing ORF, and a chemical compound which should affect the growth of the strain, if added as a nutrient to its environment. This level of hypothesis is derived from the first by logical inference using our model of yeast metabolism. An example of such a hypothesis is: affects growth('C00108','YBR166C'), where the first argument is the compound (names according to KEGG) and the second argument is the deletant strain. More examples of Adam's hypotheses can be found on our website (see appendix note 1). Adam then designs the experimental assays required to test these hypotheses for execution on the laboratory robotic system. These experiments are based on a two-factor design that compares multiple replicates of deletant strains with and without metabolites compared against wild type strain controls with and without metabolites. Full details of the experiment design process (such as how suitable metabolites were chosen) can be found on the Robot Scientist website (see appendix note 1).
The measurements observed by the system are optical density measurements taken at 595 nm recorded by the two microtitre plate readers (see appendix note 3), which when plotted over time become graphs that act as a proxy for cellular growth and indicate phenotype. The growth curves are smoothed, after which biologically significant parameters are extracted and statistically analysed to determine whether the original hypotheses have been confirmed or refuted. Scientific knowledge gained from this process is used to update the model of yeast metabolism. Full details about all these processes can be found on the Robot Scientist website (see appendix note 1).
The yeast S. cerevisiae is extensively studied as a model for eukaryotic cells. Yeast has a small physical size and fast generation time which makes it suitable for high-throughput experimentation, plus the growth curve results are relatively easy to observe and highly sensitive to changes in genotype and environment. There already exists a vast amount of information about yeast, including a detailed (but still incomplete) logical model of its metabolic pathways . In Adam this model was used both as the basis for forming hypotheses, and also when designing the experiments required to test these hypotheses .
Adam is intended to be fully automated, with human intervention required only to supply library strain stocks and consumables. As such, Adam includes a collection of software components that together allow the system to perform cycles of experimentation.
In Adam's early work, the Inductive Logic Programming program C-Progol 5 [27, 28] was used to automatically infer hypotheses for the investigation of aromatic amino acid metabolism. The inference was a restricted form of Abductive Logic Programming , where an incomplete background theory and experimental observations were used to infer facts concerning gene function. A logic program corresponding to the Aromatic Amino Acid Biosynthesis pathway of S. cerevisiae was used as the background theory and the inferred facts matched an ORF from yeast to a catalysing enzyme. The hypotheses completed the background theory by rediscovering the missing ORF/enzyme relations.
Identify Enzyme Commission (E.C.) numbers corresponding to enzymes which participate in yeast metabolism but have no known ORF assigned to them.
For each E.C. number find the ORFs in other organisms that code for that enzyme. Use all organisms from the KEGG genome database for this search. Collect all amino acid sequences for these ORFs. These are known as the 'query sequences'.
For each query sequence use sequence similarity search (PSI-BLAST or FASTA) to identify the most similar sequences/ORFs in S. cerevisiae.
A single hypothesis is the mapping of one S. cerevisiae ORF to one E.C. class - e.g. YER152C → 18.104.22.168. There are typically many hypotheses for each enzyme class.
Experiment design code then uses the system model to generate biological experiment plans involving deletant strains and metabolite solutions to test the hypotheses, creating microplate layouts using Latin-square design to improve the detection of quantitatively small differences above the background noise. The microplate layouts and related liquid handler volume files are passed to the robotic system control software, that executes the experiment plans. The resulting growth curve data is processed using algorithms based on cubic splines to fit, smooth and de-noise the curves, and then to extract biologically significant parameters such as growth rate and lag time. The parameters from multiple experiment replicates are analysed using machine learning (random forests ) to obtain statistically significant results that can be used to either confirm or refute hypotheses, potentially resulting in new scientific knowledge that can be used to update the system model. The cycle can then repeat with further hypothesis generation.
Finally, there is a comprehensive custom-made relational database that stores all the data and meta-data generated throughout the various stages (MySQL).
Further details describing all the software and informatics can be found online at our website (see appendix note 1).
Adam conceived 20 hypotheses concerning the identity of genes encoding 13 locally orphan enzymes in S. cerevisiae. Adam tested all these hypotheses on its robotic system and was able to confirm by experimentation, with a high degree of confidence, the correctness of 12 of them . Conventional manual biological experiments were performed to verify 3 of these conclusions, and additional detailed literature searches revealed evidence supporting a further 6 more. Subsequent comparative genomics also indicated a number of possible reasons why the identities of some of the genes encoding these locally orphan enzymes had remained unknown for so long: there appear to have been gene duplication events with retained overlapping functions, a number of the enzymes appear to catalyse more than one associated reaction, and some of the functional annotations in the existing literature are incorrect. Adam's use of bioinformatic and quantitative phenotypic analyses were needed to deconvolve this functional complexity. Interestingly, Adam also came to an incorrect conclusion regarding one of its original 20 hypotheses which highlights a weakness in its system model. This was because the hypothesised gene candidate YIL033C was predicted to be a glutaminase enzyme, and Adam confirmed this activity experimentally by performing assays involving 11 metabolites predicted to have differential effect on a glutaminase deletant. However, it transpires that YIL033C has a cAMP-dependent protein kinase sub-unit which is involved in regulating metabolism, and this could also explain the observed phenotype. Adam's current metabolism system model does not represent kinase control mechanisms, and so Adam did not take this into account. There is also the possibility that YIL033C is both a kinase and a glutaminase, and some evidence exists to support this theory (see ).
See the Robot Scientist website for more details on Adam's results, its hardware and its software (see appendix note 4).
We formalised the information related to Adam's investigations. This was based on the generic ontology of scientific experiments: EXPO [32, 33]. We developed a custom version of EXPO called LABORS (see appendix note 5) which was tailored to formalise Adam's experiments. We also developed an ontology to describe experimental actions (both by humans and machines) called EXACT .
LABORS was developed when no generic formalism for the logical description of experiments was available. The OBI project aims to provide such a formalism and the first release is due in the near future . The Robot Scientist project joined the OBI consortium in October 2008, and the LABORS representations which are common to other biological domains have been aligned with the OBI representations. However, other terms specific to automated investigations still remain within LABORS. Use of LABORS resulted in the generation of a nested tree structure 10 levels deep containing over 10,000 research elements, connecting all the experimental information to the observations. These data are expressed in the logical programming language Datalog , and have been made publicly available (see the 'Results' section on the Robot Scientist website - see appendix note 4). This explicit logical description makes Adam's investigations more explicit, reproducible, and reusable.
A Robot Scientist to study chemical genetics and drug design - 'Eve'
The main goal with Eve is to integrate machine learning and automated quantitative structure-activity relationship (QSAR ) into the drug-screening process, to improve both the efficiency and quality, as well as reduce the cost, of a primary drug screen. Eve will begin by performing a standard mass screen against the target assay, monitoring the results in real time, and when sufficient hits are found it will stop the mass screen. After verifying the hits Eve will then switch to a more targeted approach using machine learning and QSARs to look at the chemical structures of the hit compounds, and generate hypotheses about what it considers would be useful compounds to test next. It then plans the screening experiments to test these hypotheses, runs these experiments on the robotic system, uses machine learning to analyse these results, and then iteratively cycles around testing other compounds until it can identify the best set of lead compounds for the target. Eve will first test those compounds which are available from its own compound library, then suggest other compounds that are commercially available that should be tested. Potentially Eve could even suggest new compounds that should be synthesised for testing.
Eve will initially use an automation-accessible compound library of 14,400 chemical compounds: the Maybridge 'Hit-finder' library (see appendix note 6). This compound library is cluster-based and was developed specifically to contain a diverse range of compounds. It was selected as a subset of the full Maybridge compound library by a two-stage filtering process based first on 'Lipinski's rule of five'  to reduce the set to 200,000 compounds, then secondly by using a Pharmacophore Fingerprinting process  and cluster analysis to further reduce the set to 14,400 compounds. We realise that this is not a large compound library by industrial standards; a pharmaceutical company may have many hundreds of thousands or even millions of compounds in its primary screening library. Our aim is to demonstrate the proof-of-principle that incorporating machine learning and QSARs into the screening process can improve on the current mass screening approach.
In addition, Eve is also physically connected to Adam via a linear track slide which allows the transfer of microtitre plates in either direction. This will allow development of assays using equipment from both systems and further increases the flexibility available in experimental design.
As with our other Robot Scientist Adam, Eve is also intended to be fully automated. Eve will include software components that will allow the system to perform cycles of targeted drug screening. There are three stages to this approach; mass screening, hit verification, and hypothesis-driven targeted screening. For each of these stages there will be experiment design code that generates the biological experiment plans, which combine chimeric yeast target strains and chemical compounds.
First, the mass screening experiment plans will be generated and passed to the robotic system control software (see appendix note 10) for execution. Monitoring software will automatically analyse the mass screening data in real time to identify and quantify chemical 'hits', and when there are sufficient hits, the system will be switched into the verification mode.
Experiment generation code will then create the verification microplate layouts to re-screen each hit against the target at multiple concentrations and with multiple replicates. Once these have been executed on the robotic system, the resulting dose-response curves will be analysed by curve smoothing algorithms and statistical tests to create a refined list of verified hits that includes quantitative information about how each chemical affected the target.
This list will then be passed to machine learning code that uses the quantitative information and QSARs to inspect the chemical structures of the hits and create hypotheses about other possibly active compounds. Cycles of targeted screening then commence to test these hypotheses, with the experiment planning code generating microplate layouts and executing them on the robotic system. The aim is for the machine learning code to analyse each successive cycle of this targeted screening and progressively refine an optimal list of lead compounds.
Eve will use the same relational database that was developed for Adam to store all its data and meta-data, with some modifications specific to the drug discovery research and storing additional instrument meta-data.
Formalisation of Eve
As with Adam, the intention is to formalise all data and meta-data relating to Eve, again by creating a derivation of the EXPO ontology. There are many similarities between Adam and Eve's data and meta-data, as well as in the types of instruments used on the systems, so we plan to identify the common elements in both the LABORS and new Eve ontologies, and update EXPO to include this common understanding.
The immediate future for our Robot Scientist work involves the continued development of the new Eve system and the improvement and continued use of the Adam system. Additionally, the two systems will be capable of working together to address scientific questions. By combining the functionality of the instrumentation on both systems we will be able to discover more about yeast, bacteria and C. elegans by preparing experiments that measure a variety of phenotypes including growth, fluorescent protein expression, cellular morphology, chemical susceptibility, growth competition, multiple gene deletions, and other visible phenotypic assays. For example, Adam's robotic system could prepare plates containing multiple cellular strains that could then be passed to Eve's robotic system where multiple compounds could be added before visualisation of the morphological effects using the automated cellular imager. The longer-term aim is for Robot Scientists to become more commonplace in the laboratory: automating reasoning, decision making and information management, as well as automating the execution of experimental procedures. We believe that such automation is necessary to meet the challenges of 21st century biological research.
Any automated scientific discovery system or Robot Scientist clearly has both advantages and disadvantages. Some of the main points are discussed here.
It has been suggested that systems such as ours would be better described as 'Laboratory Assistants' rather than the implicitly more independent term 'Scientists'. Although in some ways the term 'Laboratory Assistant' has some merit, as they are not independent workers. In other, more important ways, the term is inappropriate as Laboratory Assistants do not generally form hypotheses, decide on the experiments to test them, automatically analyse and interpret the results etc. It should also be remembered that these systems are still just prototypes, and it is probable that future developments in hardware and software will increase the independent nature of such systems. So, on balance, we prefer the more evocative term 'Robot Scientist', and argue that the Adam system has discovered new knowledge about gene function in S. cerevisiae that has been independently verified .
Another common argument against Robot Scientists is that they remove the chance for serendipitous discovery, and that they are incapable of innovation. We would argue that more often than not a serendipitous discovery is simply the result of an experiment that has been designed without prior analysis of all the potential outcomes. Louis Pasteur phrased this sentiment as 'In the fields of observation chance favours only the prepared mind'. While it is true that the underlying artificial intelligence components fail to meet human expectations for innovative thought, we believe that developing a richer background model and incorporating more sophisticated reasoning mechanisms will bring us closer to that goal.
It is also true that a Robot Scientist generates its hypotheses based on information obtained from publicly available databases, and as such is susceptible to any errors contained therein. However, this is no different to the situation in which human scientists find themselves, as they also have to rely on published information. Most such databases are curated by humans, and provide a service that biologists routinely use in their work. Both could choose (or in the case of the Robot Scientist be programmed) to assign weightings in their confidence of various pieces of evidence based on how they were labelled in the databases (e.g. indirect vs direct experimental evidence). When errors are present the robot is most likely to propose an incorrect hypothesis which the experimental data will then refute. For Adam we avoided problems by primarily using only one public database (KEGG) and manually updating our system model where conflicts were noticed, before allowing automated generation of hypotheses. Where a Robot Scientist may have more trouble is where it lacks the broader background knowledge base which may be available to a human scientist (e.g. the problem with Adam's system model not representing kinases mentioned earlier). A better system model and a broader knowledge base can be developed for Robot Scientists that in time would negate this difference.
Similarly, it has been pointed out that the data analysis algorithms of a Robot Scientist might be less able to deal with flaws in experimental measurements than a human, and may come to false conclusions as a consequence. We believe this to be mostly a matter of refinement of programming; for example, Adam's growth curve smoothing and de-noising routines use machine learning and statistical data analysis of multiple replicates to routinely deal with the effects of significant noise, contamination, and even gaps in the measured readings. Further refinement could be done to identify abnormal or other unexpected results, for example the shape of a bacterial contamination growth curve, or the long lag time associated with yeast cross-contamination, and then discount them automatically. The advantage of a Robot Scientist here is that it would always be consistent in its handling of the data. More physical issues with an experiment, for example flaws in plastic wares, faults in instruments, incorrect placements of plates in instruments etc. are currently easier for a human to notice and correct. Whilst there are some measures we can put in place to automatically deal with this type of issue (e.g. calibration and fault detection system checks prior to experiment runs), we believe that future refinements of the hardware and plastic wares used in laboratory automation will reduce the effects of this type of problem.
Finally, there has also been discussion about costs, comparing the cost of using a Robot Scientist against using humans to perform the same tasks. There is a substantial cost for these systems, not only in initial capital outlay and user training, but also in ongoing servicing and maintenance costs, and we would not currently consider them to be 'cost-effective' in comparison to human scientists. However, these systems are early prototypes, and we would expect such costs to reduce significantly as laboratory automation becomes more widespread, more reliable, and the software more user-friendly. The cost of hiring human scientists and technicians and buying the instruments and equipment they need to perform such high-throughput and complex experiments should not be underestimated either, and the robots have the advantages of efficiency, consistent quality, and the ability to run outside normal working hours.
Robot Scientists are the next logical step in laboratory automation. They can automate all aspects of the scientific discovery process: they generate hypotheses from a computer model of the domain, design experiments to test these hypotheses, run the physical experiments using robotic systems, and then analyse and interpret the results. They also have the potential to record every detail of what they have done and why, enabling scientific investigations to be more reproducible and reuseable. We look forward to a time when Robot Scientists will commonly work with human scientists to progress the path of science.
Robot Scientist informatics, http://www.aber.ac.uk/compsci/Research/bio/robotsci/data/informatics/
Sciclone i1000, Caliper Life Sciences, Hopkinton, MA, USA
Spectramax 190 readers, MDS Analytical Technologies, Concord, Ontario, Cananda
Robot Scientist website, at http://www.aber.ac.uk/en/cs/research/cb/projects/robotscientist/
Maybridge Hitfinder library, Maybridge, Cornwall, UK
Echo 550 acoustic liquid handler, Labcyte, Sunnyvale, CA, USA
Pherastar and Polarstar readers, BMG labtech BmgH, Offenburg, Germany
ImageXpress Micro, MDS Analytical Technologies, Concord, Ontario, Cananda
Overlord 2 control software, PAA Ltd., Farnborough, UK
Thanks to the reviewers for their helpful suggestions and comments.
Thanks also to our laboratory technicians for all their hard work maintaining and supplying the robotic systems; Magdalena Markham, Katherine Martin and Ronald Pateman.
This work was funded by the U.K. Biotechnology and Biological Sciences Research Council grants, by SRIF 2 and SRIF 3 awards from the Higher Education Funding Council for Wales, and by fellowships from the Royal Academy of Engineering/Engineering and Physical Sciences Research Council, and the Royal Commission for the Great Exhibition of 1851 for AC, and from Research Councils UK for LS.
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